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FERTILITY LIBRARY

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History of Saliva Crystallization Test

1945:	Papanicolau first observed ferning (crystal formation) in dried cervical fluid
1969:	Dr. Biel Cassals, a Spanish gynecologist, studied the crystallization of saliva and concluded that the ferning of saliva is virtually identical in appearance to the arborization effect of cervical fluid.
1991:	M. Guida, Institute of Gynecology and Obstetrics Clinic in Napoli, Italy, study achieved a 92% positive result matching saliva ferning to the fertile preovulatory and ovulatory period.
1992:	M. Barbato, A. Pandolfi, M. Guida study found direct correlation between saliva ferning and the fertile period, stating that “salivary ferning may be used as a new parameter to aid women to detect the fertile period in combination with other symto-thermal methods of ovulation detection.”
1992:	2nd Department of Gynecology & Obstetrics in Brno, Czechoslavakia studied 300 women from an in vitro fertilization program who used a handheld microscope device to track saliva ferning and ovulation period. Results from the study determined a definite correlation between estrogen activity and the crystallization of saliva, classifying the reliability as “very high level”. When combined with the basil temperature method, a 99% reliability rate was claimed.
1994:	Clinical Center of Serbia conducted a study, “Determination of Fertile and Infertile Days of the Menstrual Cycle of Women by Using the Saliva Crystallization Test”, and found saliva ferning to be as effective as cervical mucus analysis and ultrasonographic folliculometry.
2001:	MaybeMOM, Inc. sponsors the first-ever U.S. clinical study in compliance with United States FDA requirements to test the effectiveness of its Mini Ovulation Microscope saliva fertility tester versus a urine-based ovulation tester. A 98% accuracy rate is achieved and the results are submitted to uphold their FDA 510(k).
2002:	FDA clearance to market the MaybeMOM Mini Ovulation Microscope in the United States is granted.

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